shuttle plasmid (pSET1 or pVA838) construct that contains the practical duplicate from the mutated gene fused in-body to the cloned S. suiswas linearized which has a restriction endonuclease (ScaI or NdeI) after which you can co-incubated Together with the protein parts of interest. The predicted oriT2 that doesn't naturally consist of 89K, was eng… Read More